Thin Sectioning of Slice Preparations for Immunohistochemistry
The present method allows reproducible cryostat sectioning of small, difficult-to-manage, tissue pieces, such as biopsies and brain slices. We utilize a simple aluminum freezing stage to facilitate handling of tissue and a standard cryostat to routinely produce 5-10 micron serial sections from 400...
Autor principal: | |
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Formato: | |
Idioma: | Inglés |
Publicado: |
Cambridge, MA :
MyJoVE Corp
2016.
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Colección: | JOVE Video Journal Biology and Medicine.
Biology. |
Acceso en línea: | Acceso a vídeo desde UNAV |
Ver en Universidad de Navarra: | https://innopac.unav.es/record=b42108391*spi |
Sumario: | The present method allows reproducible cryostat sectioning of small, difficult-to-manage, tissue pieces, such as biopsies and brain slices. We utilize a simple aluminum freezing stage to facilitate handling of tissue and a standard cryostat to routinely produce 5-10 micron serial sections from 400 micron thick brain slices. |
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Notas: | Tít. sacado de la página de descripción del recurso. |
Descripción Física: | 1 recurso electrónico (492 seg.) : son., col |
Formato: | Forma de acceso: World Wide Web. |
Público: | Para estudiantes universitarios, graduados y profesionales. |