Protocol for RNAi Assays in Adult Mosquitoes (A. gambiae)

Reverse genetic approaches have proven extremely useful for determining which genes underly resistance to vector pathogens in mosquitoes. This video protocol illustrates a method used by the Dimopoulos lab to inject dsRNA into Anopheles gambiae mosquitoes, which harbor the malaria parasite. The t...

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Detalles Bibliográficos
Autor principal: Garver, Lindsey (-)
Formato: Video
Idioma:Inglés
Publicado: Cambridge, MA : MyJoVE Corp 2016.
Colección:JOVE Video Journal Biology and Medicine.
Biology.
Acceso en línea:Acceso a vídeo desde UNAV
Ver en Universidad de Navarra:https://innopac.unav.es/record=b42108068*spi
Descripción
Sumario:Reverse genetic approaches have proven extremely useful for determining which genes underly resistance to vector pathogens in mosquitoes. This video protocol illustrates a method used by the Dimopoulos lab to inject dsRNA into Anopheles gambiae mosquitoes, which harbor the malaria parasite. The technique manipulating the injection setup and injecting dsRNA into the thorax is illustrated.
Notas:Tít. sacado de la página de descripción del recurso.
Descripción Física:1 recurso electrónico (708 seg.) : son., col
Formato:Forma de acceso: World Wide Web.
Público:Para estudiantes universitarios, graduados y profesionales.