Primary Culture and Plasmid Electroporation of the Murine Organ of Corti
This procedure describes a method for the isolation and culture of the murine organ of Corti with or without the spiral limbus and spiral ganglion neurons. We also demonstrate a method for the expression of an exogenous reporter gene in the organ of Corti explant by electroporation.
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Formato: | |
Idioma: | Inglés |
Publicado: |
Cambridge, MA :
MyJoVE Corp
2016.
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Colección: | JOVE Video Journal Biology and Medicine.
Biology. |
Acceso en línea: | Acceso a vídeo desde UNAV |
Ver en Universidad de Navarra: | https://innopac.unav.es/record=b42103873*spi |
Sumario: | This procedure describes a method for the isolation and culture of the murine organ of Corti with or without the spiral limbus and spiral ganglion neurons. We also demonstrate a method for the expression of an exogenous reporter gene in the organ of Corti explant by electroporation. |
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Notas: | Tít. sacado de la página de descripción del recurso. |
Descripción Física: | 1 recurso electrónico (749 seg.) : son., col |
Formato: | Forma de acceso: World Wide Web. |
Público: | Para estudiantes universitarios, graduados y profesionales. |