A Fluorescence Microscopy Assay for Monitoring Mitophagy in the Yeast Saccharomyces cerevisiae

A robust approach to monitor the delivery of organelles to the acidic lumen of the yeast vacuole for degradation and recycling is described. The method relies on the specific labeling of target organelles with a genetically encoded dual-emission fluorescence pH-biosensor, and visualization of indivi...

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Detalles Bibliográficos
Autor principal: Mijaljica, Dalibor (-)
Formato: Video
Idioma:Inglés
Publicado: Cambridge, MA : MyJoVE Corp 2016.
Colección:JOVE Video Journal Biology and Medicine.
Biology.
Acceso en línea:Acceso a vídeo desde UNAV
Ver en Universidad de Navarra:https://innopac.unav.es/record=b42100239*spi
Descripción
Sumario:A robust approach to monitor the delivery of organelles to the acidic lumen of the yeast vacuole for degradation and recycling is described. The method relies on the specific labeling of target organelles with a genetically encoded dual-emission fluorescence pH-biosensor, and visualization of individual cells using fluorescence microscopy.
Notas:Tít. sacado de la página de descripción del recurso.
Descripción Física:1 recurso electrónico (627 seg.) : son., col
Formato:Forma de acceso: World Wide Web.
Público:Para estudiantes universitarios, graduados y profesionales.