Generation of High Quality Chromatin Immunoprecipitation DNA Template for High-throughput Sequencing (ChIP-seq)

The combination of chromatin immunoprecipitation and ultra-high-throughput sequencing (ChIP-seq) can identify and map protein-DNA interactions in a given tissue or cell line. Outlined is how to generate a high quality ChIP template for subsequent sequencing, using experience with the transcription f...

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Detalles Bibliográficos
Autor principal: Deliard, Sandra (-)
Formato: Video
Idioma:Inglés
Publicado: Cambridge, MA : MyJoVE Corp 2016.
Colección:JOVE Video Journal Biology and Medicine.
Biology.
Acceso en línea:Acceso a vídeo desde UNAV
Ver en Universidad de Navarra:https://innopac.unav.es/record=b42095037*spi
Descripción
Sumario:The combination of chromatin immunoprecipitation and ultra-high-throughput sequencing (ChIP-seq) can identify and map protein-DNA interactions in a given tissue or cell line. Outlined is how to generate a high quality ChIP template for subsequent sequencing, using experience with the transcription factor TCF7L2 as an example.
Notas:Tít. sacado de la página de descripción del recurso.
Descripción Física:1 recurso electrónico (592 seg.) : son., col
Formato:Forma de acceso: World Wide Web.
Público:Para estudiantes universitarios, graduados y profesionales.