Protocols for Implementing an Escherichia coli Based TX-TL Cell-Free Expression System for Synthetic Biology
This five-day protocol outlines all steps, equipment, and supplemental software necessary for creating and running an efficient endogenous Escherichia coli based TX-TL cell-free expression system from scratch. With reagents, the protocol takes 8 hours or less to setup a reaction, collect, and proces...
Autor principal: | |
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Formato: | |
Idioma: | Inglés |
Publicado: |
Cambridge, MA :
MyJoVE Corp
2016.
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Colección: | JOVE Video Journal Biology and Medicine.
Biology. |
Acceso en línea: | Acceso a vídeo desde UNAV |
Ver en Universidad de Navarra: | https://innopac.unav.es/record=b42093703*spi |
Sumario: | This five-day protocol outlines all steps, equipment, and supplemental software necessary for creating and running an efficient endogenous Escherichia coli based TX-TL cell-free expression system from scratch. With reagents, the protocol takes 8 hours or less to setup a reaction, collect, and process data. |
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Notas: | Tít. sacado de la página de descripción del recurso. |
Descripción Física: | 1 recurso electrónico (971 seg.) : son., col |
Formato: | Forma de acceso: World Wide Web. |
Público: | Para estudiantes universitarios, graduados y profesionales. |