Enrichment for Chemoresistant Ovarian Cancer Stem Cells from Human Cell Lines
Cancer stem cells (CSCs) are implicated in tumor relapse due to chemoresistance. We have optimized a protocol for selection and expansion of CSCs from ovarian cancer cell lines. By treating cells with the chemotherapeutic cisplatin and culturing in a stem cell promoting media we enrich for non-adher...
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Formato: | |
Idioma: | Inglés |
Publicado: |
Cambridge, MA :
MyJoVE Corp
2016.
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Colección: | JOVE Video Journal Biology and Medicine.
Medicine. |
Acceso en línea: | Acceso a vídeo desde UNAV |
Ver en Universidad de Navarra: | https://innopac.unav.es/record=b42090052*spi |
Sumario: | Cancer stem cells (CSCs) are implicated in tumor relapse due to chemoresistance. We have optimized a protocol for selection and expansion of CSCs from ovarian cancer cell lines. By treating cells with the chemotherapeutic cisplatin and culturing in a stem cell promoting media we enrich for non-adherent CSC cultures. |
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Notas: | Tít. sacado de la página de descripción del recurso. |
Descripción Física: | 1 recurso electrónico (710 seg.) : son., col |
Formato: | Forma de acceso: World Wide Web. |
Público: | Para estudiantes universitarios, graduados y profesionales. |