A Simplified System for Evaluating Cell Mechanosensing and Durotaxis In Vitro
Many mammalian cells preferentially migrate towards a more rigid matrix or substrate through durotaxis. The goal of this protocol is to provide a simple in vitro system that can be used to study and manipulate cell durotaxis behaviors by incorporating polydimethylsiloxane (PDMS) substrates of define...
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Formato: | |
Idioma: | Inglés |
Publicado: |
Cambridge, MA :
MyJoVE Corp
2016.
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Colección: | JOVE Video Journal Biology and Medicine.
Biology. |
Acceso en línea: | Acceso a vídeo desde UNAV |
Ver en Universidad de Navarra: | https://innopac.unav.es/record=b42086449*spi |
Sumario: | Many mammalian cells preferentially migrate towards a more rigid matrix or substrate through durotaxis. The goal of this protocol is to provide a simple in vitro system that can be used to study and manipulate cell durotaxis behaviors by incorporating polydimethylsiloxane (PDMS) substrates of defined rigidity, interfacing with glass coverslips. |
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Notas: | Tít. sacado de la página de descripción del recurso. |
Descripción Física: | 1 recurso electrónico (590 seg.) : son., col |
Formato: | Forma de acceso: World Wide Web. |
Público: | Para estudiantes universitarios, graduados y profesionales. |