Microbial Proteomics
| Otros Autores: | |
|---|---|
| Formato: | Libro electrónico |
| Idioma: | Inglés |
| Publicado: |
Singapore :
Bentham Science Publishers
2020.
|
| Colección: | EBSCO Academic eBook Collection.
Current and Future Developments in Proteomics. |
| Acceso en línea: | Conectar con la versión electrónica |
| Ver en Universidad de Navarra: | https://innopac.unav.es/record=b46109651*spi |
Tabla de Contenidos:
- Cover
- Title
- Copyright
- End User License Agreement
- Contents
- Foreword
- Preface
- ACKNOWLEDGEMENTS
- About the Editor
- Dedication
- List of Contributors
- Microbial Biofilm and Drug Resistance: A Proteomic Approach
- Sarika Sharma1 and Sandeep Sharma2,*
- INTRODUCTION
- The Process of Biofilm Formation
- Attachment
- Micro-Colony Formation
- Detachment
- Mechanism of Biofilm Resistance against Antimicrobials
- Limited Penetration of Drugs Due to Glycocalyx
- Enzyme-Mediated Resistance
- State of Metabolism and Rate of Growth in Biofilms
- Genetic Adaptations.
- Efflux Pumps
- Quorum Sensing
- TARGET PROTEINS TO STUDY BIOFILM
- Proteins Involved in Cellular Metabolism
- Transporter Proteins
- Stress-responsive Proteins
- Proteomic Techniques to Study Biofilm
- Stable Isotope Labeling by Amino Acids in Cell Culture (SILAC)
- Isotope-coded Affinity Tags (ICAT)
- Isobaric Tags for Relative and Absolute Quantification (iTRAQ)
- Tandem Mass Tags (TMT)
- Isotope-coded Protein Label (ICPL)
- Bioorthogonal Noncanonical Amino Acid Tagging (BONCAT)
- Future Prospectus
- CONCLUSION
- CONSENT FOR PUBLICATION
- CONFLICT OF INTEREST
- ACKNOWLEDGEMENTS.
- REFERENCES
- Past, Present, and Future of Gel-Based Microbial Proteomics
- Munna Lal Yadav1,*, Arvind K. Verma1, Preeti Rawat1, Abhishek Parashar1, Divakar Sharma2, Sudarshan Kumar1 and Ashok K. Mohanty1,*
- INTRODUCTION
- PROTEIN SAMPLE PREPARATION FROM MICROBIAL SOURCE
- CLEAN-UP AND QUANTIFICATION OF PROTEINS
- GEL ELECTROPHORESIS OF PROTEINS
- TYPES OF PAGE
- SDS-PAGE (Sodium Dodecyl Sulphate-polyacrylamide Gel Electrophoresis)
- Tricine SDS-PAGE
- Native Page
- SDD-AGE (Semi Denaturing Detergent-agarose Gel Electrophoresis)
- 2D-PAGE (Two-dimensional Gel Electrophoresis).
- Isoelectric Focusing (First Dimension)
- SDS-PAGE (Second Dimension)
- 2D-DIGE (TWO DIMENSIONAL DIFFERENCE IN-GEL ELECTROPHORESIS)
- ANALYSIS OF 2D GEL AND DIGE GEL BY DIFFERENT SOFTWARE
- 2D Gel Analysis using Image Master 2D Platinum
- Evaluation of DIGE Gels using DeCyder
- COMPARISON BETWEEN IEF, SDS-PAGE, 2D-PAGE, AND DIGE
- MICROBIAL PROTEOMICS IN DAIRY PROCESSING
- FUTURE PERSPECTIVES OF GEL BASED MICROBIAL PROTEOMICS
- CONSENT FOR PUBLICATION
- CONFLICT OF INTEREST
- ACKNOWLEDGEMENTS
- REFERENCES.
- Liquid Chromatography-Mass Spectrometry (LCMS): An Advanced Tool for the Microbial Proteomics Analysis
- Pranav Kumar Prabhakar*
- INTRODUCTION
- MASS SPECTROSCOPY (MS)
- Shotgun MS-Based Proteomics
- Shotgun MS-Based Quantitative Proteomics
- EXPERIMENTAL PROCEDURE
- i. Sample Preparation
- ii. Mass Spectrometry
- DATA ACCOMPLISHMENT
- PROTEIN IDENTIFICATION
- USE OF MALDI-TOF IN MICROBIAL IDENTIFICATION AND ANTIMICROBIAL RESISTANCE
- CONCLUSION
- CONSENT FOR PUBLICATION
- CONFLICT OF INTEREST
- ACKNOWLEDGEMENTS
- REFERENCES.
