Primary Tumor and MEF Cell Isolation to Study Lung Metastasis

The goal of this protocol is to study breast tumorigenesis. With this technique, mouse mammary tumors are removed and primary cells are prepared from tumors. A lung extraction protocol is included for studying lung metastasis. Furthermore, another protocol for analyzing mouse embryonic fibroblasts f...

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Detalles Bibliográficos
Autor principal: Dong, Shengli (-)
Formato: Video
Idioma:Inglés
Publicado: Cambridge, MA : MyJoVE Corp 2016.
Colección:JOVE Video Journal Biology and Medicine.
Medicine.
Acceso en línea:Acceso a vídeo desde UNAV
Ver en Universidad de Navarra:https://innopac.unav.es/record=b4208717x*spi
Descripción
Sumario:The goal of this protocol is to study breast tumorigenesis. With this technique, mouse mammary tumors are removed and primary cells are prepared from tumors. A lung extraction protocol is included for studying lung metastasis. Furthermore, another protocol for analyzing mouse embryonic fibroblasts from the mouse embryo is included.
Notas:Tít. sacado de la página de descripción del recurso.
Descripción Física:1 recurso electrónico (391 seg.) : son., col
Formato:Forma de acceso: World Wide Web.
Público:Para estudiantes universitarios, graduados y profesionales.