Current Laboratory Methods in Neuroscience Research

Current Laboratory Methods in Neuroscience Research is a research manual for both students and seasoned researchers. It focuses on commonly-used techniques employed in neuroscience research, presented in a simple, step-by-step manner for laboratory use. The manual also offers a zblueprinty for  benc...

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Detalles Bibliográficos
Autor Corporativo: SpringerLink (-)
Otros Autores: Xiong, Huangui (-), Gendelman, Howard E.
Formato: Libro electrónico
Idioma:Inglés
Publicado: New York, NY : Springer New York 2014.
Colección:Springer eBooks.
Acceso en línea:Conectar con la versión electrónica
Ver en Universidad de Navarra:https://innopac.unav.es/record=b32996007*spi
Tabla de Contenidos:
  • Section 1: Neurohistological techniques
  • 1. Brain tissue preparation, sectioning and staining
  • 2. Brain stereotactic injections
  • 3. Tract tracing at light and electron microscopy
  • 4. Stereological analysis of neurological tissues
  • Section 2: In vitro preparations
  • 5. Preparation and use of rodent hippocampal brain slices
  • 6.  Single-Cell Neuronal Dissociation For Electrophysiological Studies
  • 7. Isolation and Culture of Human Neurons, Microglia and Astrocytes
  • 8. Isolation and Culture of Neural Stem/progenitor Cells
  • 9. Isolation of mitochondria from brain tissue and cells
  • 10. Isolation of synaptosomes from archived brain tissues
  • 11. Xenopus oocytes isolation and microinjection
  • Section 3: Leukocyte isolation and application in neuroscience
  • 12. Centrifugal elutriation for studies of neuroimmunity
  • 13. Flow cytometry in neuroscience research
  • Section 4: Standard Laboratory Nucleic Acid and Protein Detections
  • 14. Western Blotting Technique in Biomedical Research
  • 15. Polymerase chain reaction (PCR) and Real-time RT PCR
  • Section 5: Nanomedicine
  • 16. Nanoformulations
  • 17. Neuronanomedicine
  • Section 6: Bioimaging
  • 18. Confocal imaging of nerve cells
  • 19. Magnetic resonance imaging (MRI)
  • 20. X-Ray, Positron Emission, and Single Photon Emission Tomographic Bioimaging
  • 21. Non-Invasive Neurophysiological Imaging with Magnetoencephalography
  • 22. Multiphoton Brain Imaging
  • Section 7: Neuroelectrophysiology
  • 23. Techniques for Extracellular Recordings
  • 24. zBlindy patch-clamp recordings from rodent hippocampal slices
  • 25. Whole-cell patch clamp recordings
  • Section 8: Immunohistochemistry and autoradiography
  • 26. Analysis of Receptor Binding And Quantitative Autoradiography
  • 27. Techniques in Immunohistochemistry and immunocytochemistry
  • Section 9: Analysis of Gene Expression
  • 28. Protein identification by mass spectrometry: Proteomics
  • 29. DNA Microarrays as a Tool for Neurosciences Research
  • 30. Metabolomics
  • 31. Bioinformatic Methods and Resources for Neuroscience Research
  • Section 10: Animal Models: Behavior and Pathology
  • 32. Motor function in rodent models of neurodegenerative disorders
  • 33. Humanized mice
  • 34. Animal models for PD and ALS
  • 35. Animal models for Alzheimer’s Disease
  • 36. Animal Models: Behavior and Pathology.