Standardisation In Cell And Tissue Engineering Methods And Protocols
The increased use of biodegradable synthetic or natural scaffolds combined with cells and/or biological molecules, in order to create functional replacement tissue in a damaged tissue site, has led to the need for the development of 'best practice' methods in the area of tissue engineering...
| Autor principal: | |
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| Formato: | Libro electrónico |
| Idioma: | Inglés |
| Publicado: |
Burlington :
Elsevier Science
2013.
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| Colección: | Woodhead Publishing Series in Biomaterials.
Science Direct e-books. |
| Acceso en línea: | Conectar con la versión electrónica |
| Ver en Universidad de Navarra: | https://innopac.unav.es/record=b30550579*spi |
Tabla de Contenidos:
- Cover; Standardisation in cell and tissue engineering : Methods and protocols; Copyright; Contents; Contributor contact details; Woodhead Publishing Series in Biomaterials; Foreword; Introduction; Part I Methods for cell and tissue engineering; 1 Fundamentals of cell and matrix biology for tissue engineering; 1.1 Introduction; 1.2 Extracellular matrices (ECMs); 1.3 ECM and cell interaction; 1.4 ECM and mechanical signalling; 1.5 Future trends; 1.6 Conclusion; 1.7 References; 2 Three-dimensional collagen biomatrix development and control.
- 2.1 Engineering cell-rich and matrix-rich tissues using collagen scaffolds2.2 Controlling the mechanical properties of collagen; 2.3 Architectural features: introducing elements of tissue complexity; 2.4 Future trends; 2.5 References; 3 Two- and three-dimensional tissue culture bioprocessing methods for soft tissue engineering; 3.1 Introduction; 3.2 Bioreactor configurations; 3.3 Selecting scaffold materials and architectures for your bioreactor; 3.4 Mass transfer in tissue engineering bioreactors; 3.5 Important parameters and taking measurements of bioreactor cultures.
- 3.6 Tissue engineering process design3.7 Future trends; 3.8 Conclusion; 3.9 Sources of further information and advice; 3.10 References; 4 Two-and three-dimensional tissue culture methods for hard tissue engineering; 4.1 Introduction; 4.2 Culture of bone and cartilage cells; 4.3 Cell culture parameters: bone tissue culture; 4.4 Cell culture parameters: cartilage tissue culture; 4.5 Two-dimensional tissue culture methods for hard tissues; 4.6 Two-and-a-half-and three-dimensional tissue culture methods for hard tissues; 4.7 Conclusion; 4.8 References.
- 5 Vascularisation of tissue-engineered constructs5.1 Introduction; 5.2 Growth of healthy vessels
- embryonic vasculogenesis; 5.3 Angiogenic diseases; 5.4 Angiogenesis and bone formation; 5.5 Cell sources for vascular tissue engineering; 5.6 Co-culture of cells: the interactions between angiogenesis and osteogenesis; 5.7 Strategies to induce in vitro prevascularisation; 5.8 Tubular formation; 5.9 Conclusion; 5.10 References; Part II Standards and protocols in cell and tissue engineering; 6 Standards in cell and tissue engineering; 6.1 Introduction; 6.2 How and by whom are standards produced?
- 6.3 The importance of an agreed lexicon6.4 Drivers for standardization; 6.5 How will standards help me?; 6.6 What standards currently exist in tissue engineering?; 6.7 Characterization of biomaterials and biomolecules; 6.8 Characterization of tissue scaffolds; 6.9 Characterization of cell-seeded scaffolds; 6.10 Manufacture, processing and storage; 6.11 Characterization of cells and cell-surface interactions; 6.12 Conclusion; 6.13 References; 7 Principles of good laboratory practice (GLP) for in vitro cell culture applications; 7.1 Introduction; 7.2 GLP governing bodies.
